L13. ANIMAL CELLS vs PLANT CELLS

L13. Animal cells vs Plant cells

1. Objectives

1. Identify the major components of cells.
2. Differentiate between animal and plant cells.
3. Measure dimensions of the entire cell and the nucleus.

2. Material

• Forceps
• Dropper
• Needle
• 2 watch glass
• Toothpick
• 2 slides
• 2 cover slips
• Distilled water
• Methylene blue
• Iodine
• Onion
• Glycerine

3. Procedure

Plant cells

First of all pour some distilled water into a watch glass and peel off the leaf from half a piece of onion and using forceps, pull out a piece of transparent onion peel from the leaf.

After put the epidermis in the watch glass containing distilled water and take a few drops of safranin solution  in a dropper and transfer into another watch glass.

Using a bursh or a needle, transfer the peel into the watch glass containing the dye. let this remain in the safranin solution for 30 seconds, so that the peel is stained.

Take a peel from the safranin solution and place it in the watch glass containing distilled water and take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.

Using a brush, place the peel onto the slide containing glycerine and take a cover slip and place it gently on the peel with the aid of a needle.

Remove  the extra glycerine using a cellulose paper.

And finally, view it in the microscope.

Animal cells

Fist of all, gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells and place the cells on a glass slide that has water on it.

Mix the water and the cheek cells using a needle and spread them.

After, dry the sample under the light to fix the sample on the slide and take a few drops of methylene blue solution using a dropper and add this to the mixture on the slide.

After 2-3 minutes remove any excess water and stain from the slide using cellulose paper and take clean cover slip and lower it carefully on the mixture with the aid of a needle.

Using a top of the needle, press the cover slip gently to spread the epithelial cells.

Finally, remove any extra liquid around the cover slip using cellulose paper.

4. Augments

1. Cell - 5,5 cm = 55000um

NA = 40x · 10x = 400

400 = 55000/MR  ---- MR = 137,5um

2. Nucleous - 0,7 cm = 7000um

400 = 7000/MR ---- MR = 17,5um