L19. MITOSI

L19. MITOSI

1. Material

• microscope
• slide
• coverslip
• dropper
• needle
• watch glass
• Beaker
• forceps
• filter paper
• distilled water
• orcein A and B
• water                                                                                                           This is a metaphase
• onions                                                                                                               NA = 40 x 10

2. Objective

Find the various phases of cell divisions onion.


3. Procedure

Keep a few days an onion on a beaker filled with water, so that the lower pate of the bulb, which is where the roots emerge, is in contact with water.
when the roots have grown a little, will cut about four centimeters from the tip i place it in a glass clock and we put orcein A and leave for 2 minutes.

then we take a wooden pegs and the burne heat the sample until the orcein to disappear a little. We put orcein B i put a slide cover and observed under a microscope.

                                                This is a metaphase
                                                     NA = 40 x 10

This is a metaphase                                                                      This is a anaphase    NA = 40 x 15
NA = 40 x 15